Purification of crime scene DNA extracts using centrifugal filter devices

Crime Scene or Event Scene?

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Assessment and mitigation of DNA loss utilizing centrifugal filtration devices.

Maximizing DNA recovery during its isolation can be vital in forensic casework, particularly when DNA yields are expected to be low, such as from touch samples. Many forensic laboratories utilize centrifugal filtration devices to purify and concentrate the DNA; however, DNA loss has been reported when using them. In this study, all centrifugal filtration devices tested caused substantial DNA lo...

Violent Crime Scene

A Centrifugal Gradocol Filter.

Details of a centrifugal Seitz filter for sterilizing small quantities of fluid were reported some months ago in this journal (Hilson and Elek, 1950). It was then considered that a similar filter, fitted with a gradocol membrane (Elford, 1931) for virus or bacteriophage work where an asbestos pad is not suitable, might be useful. Moreover, a suggestion has been made (Latner and Slack, 1950) tha...

Isolation of genomic DNA from blood using a novel filter-based DNA purification technology.

A novel DNA purification technology is described that enables the purification of pure dsDNA from blood. When compared to existing DNA purification methods, the Whatman BioScience Purification System (WBPS) offers a fast and convenient way to recover high yields of DNA. WBPS is based on a unique filter system that entraps DNA within a matrix. This allows the process to be performed in a single ...